Fortessa and LSR

BD LSRII BD LSRFortessa

The BD LSRFortessa™ and BD™ LSRII cell analysers are high end analytical flow cytometers and offer unmatched performance, capabilities, and flexibility, utilising a revolutionary optical design. The electronics digitise signals 10 million times per second in 16,384 discrete levels. This expands channel resolution and increases detection sensitivity. Electronic dead time is eliminated; more precise fluorescent measurements can be made improving linearity, compensation, and better quantification of fluorescence measurements.

The core lab LSR II and Fortessa are equipped with five fixed alignment lasers (488 nm, 405 nm, 633 nm, 561 nm and 355 nm) and BD High Throughput Sampler (HTS) option. The BD LSRFortessa™ and BD™ LSRII cell analysers use FACSDIVA software for acquisition and analysis.

Configuration of the BRC LSR II and Fortessa analysers

BRC SORP Fortessa and LSR II: 5 laser, 20 parameter SORP instrument (355 nm (nUV), 405 nm (violet), 488nm (blue), 561nm (Yellow-green), 633 nm (red)), allow simultaneous use of all lasers. Equipped with HTS option. Laser, detector and filter configuration 3 Blue/3 Red/5 Violet /2 nUV/ 5 Yellow-Green:

Laser	LP Mirror	BP Filter	Intended Dye
488 nm Optional	550 nm	575/26 nm	PE
	505 nm	530/30 nm	FITC
	Blank	488/10 and pinhole	SSC
	505 nm	525/50	FITC
	495 nm	515/20 nm	EGFP
	525 nm	545/35 nm	YFP
	635 nm	670 LP	PerCP, PerCP-Cy5.5
561 nm Optional	735 nm	780/60 nm	PE-Cy7
	685 nm	710/50 nm	PE-Cy5.5
	635 nm	660/20 nm	PE-Cy5
	595 nm	610/20 nm	PE-TexasRed,mCherry
	570 nm	585/15 nm	PE
	750 nm	780/60 nm	PE-Cy7
	635 nm	670/30 nm	PE-Cy5
633 nm Optional	735 nm	780/60 nm	APC-Cy7
	685 nm	710/50 nm	Alexa700
	Blank	670/14	APC
	750 nm	780/60 nm	APC-Cy7
	690 nm	730/45 nm	Alexa700
	Blank	670/30 nm	APC
405 nm	755 nm	780/60 nm	QDot 800
	690 nm	710/50 nm	QDot 700
	600 nm	610/20 nm	QDot 605
	570 nm	585/42 nm	QDot 585
	505 nm	525/50 nm	AmCyan
	Blank	450/50 nm	Pacific Blue
351 nm	505 nm	530/30	Indo1
	Blank	450/50 nm	DAPI/Hoechst

Note
Optional: The core laboratory owns these filters as well, but they are not routinely installed in the cytometer. If a user requires filters for an experiment, please inform FCL staff in advance.

Booking

The analysers are available 24 hours a day, 7 days a week for use by investigators except during weekly maintenance and repair periods. For details of these, see the guidelines page.

Before an investigator can book and use the equipment, they must attend a basic training session in order to obtain log-in credentials to the instruments and online booking calendar.

Book time on BD Fortessa

Book time on BD LSR II

When booking, please note the following:

The equipment is available in 15 minute time slots.
No more than 4 hours can be continuously booked during prime time usage (Monday - Friday, 12pm - 8pm).
No single user can book more than 8 hours of prime time usage per week.
Members of the BRC's Faculty of Translational Medicine will be given priority access.

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